HEMAGGLUTINATION

Purpose: To identify certain viruses (antigens*) that hemagglutinate chicken RBCs (e.g. NDV or Avian Influenza)

Method:

  1. Allantoic cavity fluid (possibly containing virus) from an embryonating chicken egg that had been inoculated with suspect material is removed.

  2. 0.1 ml of this fluid is placed on a glass plate and 0.1 ml of a 5.0% suspension of chicken RBC's is added.

  3. If a hemagglutinating agent is present, the RBCs will clump.

*Note - This is not a serological test. While it is used as the basis for the Hemagglutination inhibition test. It detects antigen not antibody.

 

TESTS:

HEMAGGLUTINATION

    HEMAGGLUTINATION INHIBITION

Purpose Identify a virus that hemagglutinates RBCs (NDV, Influenza) Quantitate serum antibody to a specific pathogen
(e.g. NDV, Influenza and Mycoplasma)
Antibodies will inhibit hemagglutination

Material

  1. Allantoic cavity fluid from embryonating chicken eggs inoculated with suspect material
  2. Chicken RBCs
  1. A known hemagglutination agent
  2. Test serum - unknown antibody
  3. Chicken RBCs

 

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