AVIAN SEROLOGICAL TECHNIQUES

PLATE AGGLUTINATION TEST

Purpose: To detect the presence of serum antibody (e.g. Mycoplasma gallisepticum Infection (MG), Mycoplasma synoviae (MS) and Mycoplasma meleagridis (MM), pullorum/typhoid)

Method:

1. Put one drop of sera on plate.

2. Add one drop of antigen.

3. Mix and incubate for two minutes.

4. A positive reaction shows a clumping effect and indicates exposure to an infectious agent.  It detects the presence of antibodies.

5. Always run a positive and a negative control.

AGAR GEL IMMUNODIFFUSION TEST

Purpose: To detect the presence of serum antibody (e.g. Avian Influenza (AI), Infectious Bursal Disease (IBD), Adeno and Reo)

Method: The test can be run in agar wells, on slides or petri dishes.

1. Antigen is placed in the center well. Positive control serum (antisera) is placed in wells on each side of the sample tested.A total of three samples can be tested in each pattern. The sample to be tested may be serum, plasma or yolks.

2. Dishes or slides are incubated at room temperature in a closed chamber for a minimum of 24 hours.

3. A white precipitant line will form between the antigen well and a positive sample.


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