Lab Manual Appendix

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
 

Lab Demonstrations

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
 

Objective:

To review the parasites of small animals in a host-oriented manner.

Hosts:

Checklist material covered in this review:

9 Giardia cysts

 9 oocysts of Cystoisospora spp. (2 sizes)

9 Cryptosporidium oocysts (acid fast stained)

9 Toxoplasma (cat) and Neospora (dog) oocysts 9 sporocyst of Sarcocystis.

9 Giardia trophozoites 9 Tritrichomonas trophozoites

9 Babesia spp. in a blood smear 9 Leucocytozoon in a blood smear

9 Ollulanus Adult (from stomach of a cat)

9 Ancylostoma spp. Egg

9 the typical lungworm L1 ("kinked" tail) and be able to identify to species (by host).

9 the L1 of Strongyloides spp.

9 Distinguish between and recognize the L1 of Strongyloides stercoralis, Ancylostoma sp.,

and Oslerus (Filaroides) sp. (or Aelurostrongylus sp. if from a cat) from the feces.

9 The eggs of the following Ascarids: Toxocara canis, T. cati, Toxascaris leonina,

9 The eggs of the following Trichocephalids: Trichuris vulpis, Trichuris spp. & Capillaria spp.

9 the L1 of Trichinella spiralis in a "squash-prep" of muscle.

9 Adult Dirofilaria immitis (by size and location in host)

9 Adult Spirocerca lupi (by size and location in host)

9 microfilaria (the pre-L1 stage of filariids)

9 a spiruid egg (all similar to S. lupi eggs)

9 The Knott concentration technique

9 The Filtration technique

9 An assay for heartworm antigen

9 any trematode egg (A brown egg with an operculum is considered a trematode egg or a

9 Paragonimus kellicotti eggs (the operculum surrounded by a thick ring and the size should be sufficient to identify this egg).

9 an acanthocephalan (a predilection site in the small intestine, and the presence of a anterior proboscis covered with spines coupled with the lack of suckers on the anterior end is enough to identify an adult acanthocephalan.)

9 Echinococcus spp. and Taenia spp. (Small (35 - 45 um) brown eggs with striated border)

9 Dipylidium caninum (expressed from a proglottid, they will be in packets)

Be able to identify the proglottids of:

9 Taenia spp. (When gently flattened they are square to rectangular.)

9 Dipylidium caninum (When gently flattened they pinch in at the ends - "cucumber seed" shaped.)

9 Dermanyssidae

9 Chyletidae

9 Psoroptidae

9 Sarcoptidae

9 Demodicidae

9 Use the pictorial key to identify an unknown tick specimen to the genus level.

9 Recognize Rhipicephalus, Ixodes, Dermacentor, Ambylomma ticks without using a key.

9 the suborders of flies by antennal type (Nematocera, Brachycera, Cyclorrhapha)

9 Adult flies of the family Tabanidae

9 Chewing lice and the two subgroups: Amblycera and Ishnocera

9 Sucking lice

9 prepare posterior spiracles of muscoid fly larvae and make a genus diagnosis

9 identify flea adults to species