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Lab 8 Appendix: Cestodes

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Introduction

The Cestodes (tapeworms) have no mouth, as adults they live in the small intestine of their hosts and absorb nutrients through their tegument (external surface). The tapeworm life cycle involves an intermediate host. In many species the larval stages cause more pathology than the adult worms. The Acanthocephalans adults also live in the small intestine of their hosts and lack a digestive tract. As the name suggests they have a spine covered proboscis at their anterior end (they are also known as "thorny headed worms"). Although many different species are found in wildlife, only one species is a parasite of domestic livestock (pigs).

Objectives Checklist

Be able to identify the eggs of:

  1. Echinococcus spp. and Taenia spp. (Small (35 - 45 um) brown eggs with striated border)
  2. Dipylidium caninum (expressed from a proglottid, they will be in packets)
  3. Anoplocephalids - Anoplocephala spp.and Moniezia spp. (Triangular or square eggs)

Be able to identify the proglottids of:

  1. Taenia spp.  (When gently flattened they are square to rectangular.)
  2. Dipylidium caninum (When gently flattened they pinch in at the ends - “cucumber seed” shaped.)

Be able to identify the adult of:

  1. Anoplocephala perfoliata (by the size, shape and predilection site)



Lab Exercises

Phylum PLATYHELMINTHES

Class Cestoda

Tapeworms of small animals

Diphyllobothrium latum: dog, cat, mink, seal, human - Freshwater fish life cycle

Spirometra mansonoides: cats - Frog or Watersnake life cycle

Mesocestoides: dog, cat - Arthropod/Vertebrate life cycle

Mesocestoides corti
Mesocestoides corti eggs
Mature Mesocestoides corti proglottid.
Note the paruterine organ (to the left in the photo). Gravid, "club-shaped" proglottids pass in the feces
Mesocestoides corti eggs
These eggs were expressed from a gravid proglottid. They measure 40 to 60 µm in diameter.

 

Dipylidium caninum: dog, cat - Flea life cycle

Dipylidium caninum
Dipylidium caninum eggs
Dipylidium caninum
Adult worms may reach up to 50 cm in length.
Dipylidium caninum egg packets
Each egg packet contains up to 20 eggs and within each is an onchosphere bearing 3 pairs of hooks. The egg packets pass from the dog within the gravid proglottid, from which they must be expressed for identification.
Dipylidium caninum fecal sample Dipylidium caninum proglottids on feline feces
At least 7 proglottids can be seen on this fecal specimen:
Four are near the kitty-litter and 3 others are to the right of the
kitty-litter.
These proglottids are usually noticed by the owner who will tell you they saw "rice grains" or tapeworms in the cat's stool. The segments may or may not be in the sample that is brought to you as the proglottids are motile.

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Taenia sp.: dog, cat - Small Mammal or Human/Livestock life cycle

Taenia saginata in situ
Taenia saginata
Taenia taeniaformis
Taenia saginata
Cysticerci in the heart of a cow.
Taenia saginata Cysticerci
Note
that many of the protoscoleces have everted.
Taenia taeniaformis
Adult worms

Click here to link to a video of a live Taenia taeniaformis proglottid

Echinococcus granulosus: dog - Sheep life cycle

Echinococcus granulosus
Echinococcus granulosus stained
Echinococcus granulosus in situ
Adult
Echinococcus granulosus
Stained adult
Echinococcus granulosus

Adult Echinococcus granulosus
in situ (dog small intestine)

Echinococcus granulosus cysts
Echinococcus granulosus cysts
Echinococcus granulosus
Hydatid cysts in the liver of a sheep.
Echinococcus granulosus
Hydatid cysts being removed from the liver of a human.
   
Echinococcus granulosus cyst diagram
Diagram of a hydatid cyst.
Echinococcus granulosus cyst histology
Cross section of a hydatid cyst. Host tissue can be seen in the upper left corner, then the wall of the cyst, the germinal layer and finally a daughter cyst, containing protoscoleces, floating free in the fluid-filled interior.

Click here to link to the Echinococcus granulosus life cycle.

 

Tapeworms of large animals

Moniezia expansa: sheep - Mite life cycle

Moniezia expansa Moniezia expansa adult
The adult of this large tapeworm (up to 6 m) is found in the small intestine of sheep (esp. lambs).
The intermediate host is a free-living mite.
Moniezia expansa eggs Moniezia expansa eggs
Triangular in shape, contain a "pyriform apparatus," and
measure approximately 56 to 67 µm.

 

Anoplocephala sp.: horse- Mite life cycle

Anoplocephala magna (top) and A. perfoliata (bottom) adults
Anoplocephala perfoliata adult tapeworms are found around the
ileo-cecal junction of horses.
There are "lappets" just below each sucker (best seen on the top middle worm in the photo).
Anoplocephala perfoliata egg -
measures about 65 to 85 µm.
Note the "pyriform apparatus" around the oncosphere in the center of the egg.

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Phylum ACANTHOCEPHALA

Macracanthorhynchus hirudinaceus: swine - Junebug Grub life cycle

Macracanthorhynchus hirudunaceus proboscis
Macracanthorhynchus hirudunaceus proboscis close-up
Macracanthorhynchus hirudinaceus


spiny proboscis ("thorny-head")


Macracanthorhynchus hirudinaceus


Enlargement of the hooks on the proboscis, used to anchor the worm to the gut wall.

 

Macracanthorhynchus hirudunaceus in situ Macracanthorhynchus hirudinaceus adults attached to the wall of a pig's small intestine. While similar in size to Ascaris suum, these worms, in life, are flattened and have pseudosegmentation.
Macracanthorhynchus hirudunaceus egg Macracanthorhynchus hirudinaceus egg
Note the multiple layers of shell and the larva (acanthor) inside.
These eggs measure 80 X 62 µm.

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Copyright © 2006 - University of Pennsylvania School of Veterinary Medicine, All rights reserved.
Faculty: Dr. Thomas Nolan
Students: Molly Church V'09, Diana Knight V'08, Douglas Gilson V'05, Chris Dykhouse V'04, Kimberly Mah V'00

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