Lab Manual Appendix

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
Lab 9
Lab 10
 

Lab Demonstrations

Lab 1
Lab 2
Lab 3
Lab 4
Lab 5
Lab 6
Lab 7
Lab 8
 

Objectives:

To review the parasites of large animals in a host oriented manner.

Hosts:

Appendix Lab 10 Checklist material covered in this review.

9 Giardia cysts  

9 Giardia trophozoites

9 Eimeria oocysts

9 Cryptosporidium oocysts (acid fast stained)

9 Tritrichomonas trophozoites

9 Babesia spp. in a blood smear

9 McMaster egg count to determine the EPG.

9 Fecal Egg Count Reduction Test

9 A typical "strongyle-type" egg

9 Nematodirus spp. egg

9 Haemonchus Adult (Largest abomasal nematode)

9 Ostertagia Adult (abomasal nematode - middle in size)

9 Trichostrongylus Adult (Smallest abomasal nematode)

9 Strongylus vulgaris Adult

9 Large Strongyle Adult (generic, from horse)

9 Small Strongyle Adult (generic, from horse)

9 Recognize the typical lungworm L1 ("kinked" tail) and be able to identify to species (by host).

9 Recognize Dictyocaulus spp. L1 from cattle (larva with straight tail in fresh feces).

9 Recognize the eggs of Strongyloides spp.

Be able to identify the following:

9 The eggs of the following Ascarids: Ascaris suum, Parascaris equorum

9 Adult ascarids to species.

9 The eggs of the following Trichocephalids: Trichuris spp. & Capillaria spp.

9 The eggs and adult female of the horse pinworm: Oxyuris equi.

9 Be able to identify the L1 of Trichinella spiralis in a "squash-prep" of muscle.

9 any trematode egg (A brown egg with an operculum is considered a trematode egg or a

9 Fasciola hepatica (the operculum, the size and the host should be sufficient to identify this egg).

9 Dicrocoelium dendriticum (the operculum, the size and the host should be sufficient to identify

9 Fasciola hepatica (by size, shape and location within the host).

9 Dicrocoelium dendriticum (by size, shape and location within the host).

9 Fascioloides magna (by size, shape and location within the host).

9 Paramphistomum spp. - (by size, shape and location within the host).

9 an acanthocephalan (a predilection site in the small intestine, and the presence of a anterior proboscis covered with spines coupled with the lack of suckers on the anterior end is enough to identify an adult acanthocephalan.)

9 Anoplocephalids - Anoplocephala spp. and Moniezia spp. (Triangular or square eggs)

9 Anoplocephala perfoliata (by the size, shape and predilection site)

9 Dermanyssidae

9 Chyletidae

9 Psoroptidae

9 Sarcoptidae

9 Demodicidae

9 Use the pictorial key to identify an unknown tick specimen to the genus level.

9 Recognize Rhipicephalus, Ixodes, Dermacentor, Ambylomma ticks without using a key.

9 the suborders of flies by antennal type (Nematocera, Brachycera, Cyclorrhapha)

9 Melophagus ovinus

9 Adult flies of the family Tabanidae

9 Chewing lice and the two subgroups: Amblycera and Ishnocera

9 Sucking lice

9 prepare posterior spiracles of muscoid fly larvae and make a genus diagnosis

9 identify flea adults to species