Common Problems with AI Technique

R.L. Nebel
Department of Dairy Science
Virginia Polytechnic Institute and State University
Blacksburg, VA 24061-0315

    Proper semen handling procedures from locating semen in the liquid nitrogen tank to entering the cow's reproductive tract need review periodically.  Everyone establishes a routine for handling frozen and thawed semen that hopefully does not injure sperm cells and lower conception rates.  Usually, errors made in the handling of frozen - thawed semen and the equipment used for artificial insemination are small.  However, mistakes in semen handling are frequently additive meaning their effects on semen quality will be magnified.  One common mistake that we all make is not concentrating on the task presently being performed.  A check list that I use to review and evaluate semen handling procedures includes 22 individual steps where errors can occur.  Listed below are the five areas I most often observe semen handling errors on the farm:

1.  Raising the canister containing the semen canes above the frost line of the tank (frost line is usually 4 to 5 inches from the top) and removing the semen from the cane using fingers, not tweezers.  Exposing frozen semen to elevated temperatures in the neck tube of the tank has the potential to cause sperm damage.  

2.  Improper thaw bath temperature.  Either not using a thermometer to obtain thaw bath temperature of 90 - 95 degrees F or using a thermometer that needs adjustment, thus not obtaining desired water bath temperature.  

3.  No timing of thawing.  Frozen semen should be in a 90 to 95 degrees F waterbath for a minimum of 40 seconds for proper thawing.

4.  Straw not dried completely prior to placement in the insemination rod.

5.  Straw not cut at proper distance from crimp sealed end (middle of air bubble) at a right angle across the straw to prevent semen feedback inside the sheath and insemination rod.



Copyright 1999-2001
New Bolton Center Field Service Department
Students:  Keith Javic - Class of 2003, C. Nikki Conroy - Class of 2003