Fecal Examination Using the Ethyl Acetate Sedimentation
Below is description of how to test a fecal sample using
the Ethyl Acetate Sedimentation Method:
When removed from the centrifuge, your tube will have clearly defined layers:
A. An ethyl acetate layer on top.
B. A plug of dissolved fat in the middle.
C. A layer of water.
D. A pellet of sediment at the bottom.
Because formalin fixed eggs and cysts may not float (they
may now have a specific gravity of greater than 1.2) this technique is
preferred for formalin fixed samples.
NOTE: If you did this technique
just to remove fat, you can resuspend the pellet in flotation solution,
centrifuge, and remove the material from the top of the float to examine