Filtration Method to Detect Microfilariae in Blood
1. Collect a 1 ml blood sample into EDTA or heparin and add to 10 ml lysing solution within a syringe. Mix thoroughly. (Lysing solution consists of 5.0 ml Triton X-100, 8.0 grams of NaCo3, 1 liter of water.)
2. Attach syringe to a filter unit (see drawing below). The lysed blood solution is pushed through an 8 um pore filter membrane.
3. Remove the filter from the filter holder, place it on a microscope slide and add one drop of 1:10,000 Methylene Blue Stain. Cover filter with a cover glass and examine under microscope.
